The Partnership for Urban Mobility, with the support of the Ministry for Regional Development and the Central Bohemian Region, organised the second annual CityChangers conference in Prague on 9 November 2023. This year's gathering was focused on the SmartCities concept in the field of transport and mobility. The aim of the conference was to spread a deeper awareness of this concept amongst professionals, with an emphasis on the area of transport infrastructure and mobility. During the conference, colleagues from the Czech Centres were given the opportunity to present the CYCLE UP! project to local decision-makers and cyclo-coordinators from different regions of Czechia.